Products/Services Used | Details | Operation |
---|---|---|
Peptide Synthesis> | ....-peptide biotin TRPNNNTRKSIRIGPGQAFYATGGAGK-biotin GenScript N/A TAPNNFTVKSIRIGPGAFYYMGGAGK-biotin.... GenScript N/A His-Tag biotin peptide biotin-ALDGGGGSHHHHHHHH A&A Labs LLC N/A Evans Blue dye Sigma | Get A Quote |
The development of stabilized recombinant HIV envelope trimers that mimic the virion surface molecule has increased enthusiasm for a neutralizing antibody (nAb)-based HIV vaccine. However, there is limited experience with recombinant trimers as immunogens in nonhuman primates, which are typically used as a model for humans. Here, we tested multiple immunogens and immunization strategies head-to-head to determine their impact on the quantity, quality, and kinetics of autologous tier 2 nAb development. A bilateral, adjuvanted, subcutaneous immunization protocol induced reproducible tier 2 nAb responses after only two immunizations 8?weeks apart, and these were further enhanced by a third immunizat... More