Products/Services Used | Details | Operation |
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Proteins, Expression, Isolation and Analysis> | The membranes were probed for 1 h at room temperature with anti-PE polyclonal antibody (Sigma-Aldrich, Rehovot, Israel) diluted by 1:15 000 or anti-His polyclonal antibody (GenScript, New Jersey, USA) diluted by 1:12 000 in Tris-buffered saline with 0.5% Tween-20 and 0.5% nonfat milk powder for the detection of PE and sfGFP, respectively. | Get A Quote |
Synthetic cells, artificial cell-like particles, capable of autonomously synthesizing RNA and proteins based on a DNA template, are emerging platforms for studying cellular functions and for revealing the origins-of-life. Here, it is shown for the first time that artificial lipid-based vesicles, containing the molecular machinery necessary for transcription and translation, can be used to synthesize anticancer proteins inside tumors. The synthetic cells are engineered as stand-alone systems, sourcing nutrients from their biological microenvironment to trigger protein synthesis. When pre-loaded with template DNA, amino acids and energy-supplying molecules, up to 2 × 10 copies of green fluoresc... More