Products/Services Used | Details | Operation |
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Nucleic Acid Purification & Analysis> | pETCON encodes Aga-2, the inserted gene, and a C-terminal c-myc tag for expression detection44. Grazoprevir/NS3a reader designs were synthesized and constructed in complete pETCON plasmids by Genscript. | Get A Quote |
Chemical and optogenetic methods for post-translationally controlling protein function have enabled modulation and engineering of cellular functions. However, most of these methods only confer single-input, single-output control. To increase the diversity of post-translational behaviors that can be programmed, we built a system based on a single protein receiver that can integrate multiple drug inputs, including approved therapeutics. Our system translates drug inputs into diverse outputs using a suite of engineered reader proteins to provide variable dimerization states of the receiver protein. We show that our single receiver protein architecture can be used to program a variety of cellular responses... More