| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | Expression vector and adenovirus for tagged EMCN A cDNA fragment encoding a double-tagged human EMCN protein (myc tag at the N-terminal of the full-length human EMCN after the signal peptide sequence and DDK tag at its C terminal) was synthesized by Genscript (Piscataway, NJ, USA). | Get A Quote |
We have previously shown that knockdown of endomucin (EMCN)， an integral membrane glycocalyx glycoprotein， prevents VEGF-induced proliferation， migration， and tube formation and angiogenesis . In the endothelium， VEGF mediates most of its angiogenic effects through VEGF receptor 2 (VEGFR2). To understand the role of EMCN， we examined the effect of EMCN depletion on VEGFR2 endocytosis and activation. Results showed that although VEGF stimulation promoted VEGFR2 internalization in control endothelial cells (ECs)， loss of EMCN prevented VEGFR2 endocytosis. Cell surface analysis revealed a decrease in VEGFR2 following VEGF stimulation in control but not siRNA directed against EMCN-transfected ECs. EM... More
