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Gene Synthesis> | coli codon-optimized DNA sequences encoding for pep- tides TrCBM1, PpCBM1, BaCBM2, BsCBM2, BaCBM5, ucCBM10, and PaCBM10 as well as for fluorescence marker Bs2 (evoglow®; evoxx technologies, Monheim am Rhein, Germany) and the affinity tag Strep-tag® II (WSHPQFEK) were synthesized by GenScript® (Piscataway, USA). | Get A Quote |
Poly(ethylene terephthalate) (PET) is one of the most widely applied synthetic polymers, but its hydrophobicity is challenging for many industrial applications. Biotechnological modification of PET surface can be achieved by PET hydrolyzing cutinases. In order to increase the adsorption towards their unnatural substrate, the enzymes are fused to carbohydrate-binding modules (CBMs) leading to enhanced activity. In this study, we identified novel PET binding CBMs and characterized the CBM-PET interplay. We developed a semi-quantitative method to detect CBMs bound to PET films. Screening of eight CBMs from diverse families for PET binding revealed one CBM that possesses a high affinity towards PET. Molecular... More