Products/Services Used | Details | Operation |
---|---|---|
Recombinant Antibody Services> | , 2012) Oligonucleotides Lysine to glutamine mutants Lysine to acetyl-lysine mutants SIRT1 and SIRT2 RNAi oligos GSK3b SiRNA assay Pyruvate carboxylase SiRNA assay Recombinant DNA Rat (Rattus norvegicus) Pck1 cDNA Plasmid: pET22b-SUMO Plasmid: pET22b-SUMO-Pck1 (rat) Plasmid: pET22b-ULP1 Plasmid: pCMV-HA Plasmid: pCMV-HA-Pck1-6xHis Plasmid: pCDF-Duet1 Plasmid: pCDF-Duet1-Pck1 Plasmid: P300-HA Plasmid: Acetyl-lysine tRNA synthetase Plasmid: Flag-SIRT1 Plasmid: Flag-SIRT1 H363Y Plasmid: TIP60 complex Plasmid: His tagged SIRT1 Plasmid: His tagged SIRT2 Plasmid: GSK3b-myc Software and Algorithms Table S5 Table S5 Table S5 Thermo Fisher Scientific Thermo Fisher Scientific GenScript (Esco´ s et al. | Get A Quote |
Cytosolic phosphoenolpyruvate carboxykinase (PCK1) is considered a gluconeogenic enzyme; however, its metabolic functions and regulatory mechanisms beyond gluconeogenesis are poorly understood. Here, we describe that dynamic acetylation of PCK1 interconverts the enzyme between gluconeogenic and anaplerotic activities. Under high glucose, p300-dependent hyperacetylation of PCK1 did not lead to protein degradation but instead increased the ability of PCK1 to perform the anaplerotic reaction, converting phosphoenolpyruvate to oxaloacetate. Lys91 acetylation destabilizes the active site of PCK1 and favors the reverse reaction. At low energy input, we demonstrate that SIRT1 deacetylates PCK1 and fully rest... More