Products/Services Used | Details | Operation |
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Peptide Synthesis> | Custom peptide P2 and the myc-peptide were ordered crude on resin from Genscript (Genscript, NJ, USA)....5 µg/mL biotin-myc (Biotin-Ahx-EQKLISEEDL, Genscript, NJ, USA) in PBS + 1% BSA was added. | Get A Quote |
Several azide-derivatized and fluorescently-labeled peptides were immobilized on azadibenzocyclooctyne (ADIBO)-activated slide surfaces via a strain-promoted alkyne-azide cycloaddition (SPAAC) reaction revealing excellent immobilization kinetics, good spot homogeneities and reproducible fluorescence signal intensities. A myc-peptide micro-array immunoassay showed an antibody limit-of-detection (LOD) superior to a microtiter plate-based ELISA. Bovine serum albumin (BSA) and dextran covalently attached via "click" chemistry more efficiently reduced non-specific binding (NSB) of fluorescently-labeled IgG to the microarray surface in comparison to immobilized hexanoic acid and various types of polyethylene glycol... More