Products/Services Used | Details | Operation |
---|---|---|
Gene Synthesis> | The plasmid for expression of HPV33 E2 tagged at its N-terminus with a triple-Flag (3 F) epitope was constructed by inserting a codon-optimized E2 ORF (synthesized commercially ; GenScript) between the BamHI-EcoRI sites of pCMV-3Tag-1a (Stratagene).... The 4 × E2BS compe t itor plasm id , wh ich contains 4 copies of E 2B S2 (5′ - CT CG AG A CC GT TT TA GG T CA T AC CG TT TT AG GT C AT A CC GT TT TA GG T CATACCGTTTTAGGTCATAGATCT-3′, E2BS2 in italics), was obtained by commercial gene synthesis (GenScript) and received cloned into the EcoRV site of pUC57. | Get A Quote |
The human papillomavirus (HPV) Long Control Region (LCR) encompasses the early promoter (EP) that drives transcription of the E6 and E7 oncogenes in keratinocytes and HPV-associated cancers. In this study, the transcriptional activities of the HPV33 EP from the prototype LCR and from eight variants representative of the worldwide diversity of the virus were examined in primary human keratinocytes (PHK) and in the HeLa cervical carcinoma cell line by luciferase reporter-gene assays. Remarkably, the two variations with the greatest effect on the EP in PHK were C7732G and a 79-bp deletion that were associated with high-grade cervical lesions and persistent infections, respectively, in epidemiological studi... More