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Gene Synthesis> | P28799)) with N-terminal TEV cleavage site was synthesized by GenScript and cloned into pET30Ek/LIC vector, resulting in a construct with a cleavable N-ter- minal hexa-his and S-Tag. | Get A Quote |
Sortilin is a neuronal receptor involved in transmembrane signaling, endocytosis, and intracellular sorting of proteins. It cycles through a number of cellular compartments where it encounters various acidic conditions. The crystal structure of the sortilin ectodomain has previously been determined at neutral pH. Here, we present the 3.5-Å resolution crystal structure of sortilin at pH 5.5, which represents an environment similar to that of late endosomes, where ligands are released. The structure reveals an overall distortion of the 10-bladed β-propeller domain. This distortion and specific conformational changes, caused by protonation of a number of histidine residues, render the currently kno... More