资源 » 参考数据库 » 客户发表文献

至今，GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次，处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果，再次证明GenScript 有能力帮助业内科学家Make research easy.

The evolutionarily conserved transcription factor PRDM12 controls sensory neuron development and pain perception.

Cell Cycle. 2015; 
Nagy Vanja，Cole Tiffany，Van Campenhout Claude，Khoung Thang M，Leung Calvin，Vermeiren Simon，Novatchkova Maria，Wenzel Daniel，Cikes Domagoj，Polyansky Anton A，Kozieradzki Ivona，Meixner Arabella，Bellefroid Eric J，Neely G Gregory，Penninger Jos
Products/Services Used Details Operation
Custom Vector Construction Prdm12 wild type (NM021619) and mutants (480G>C to generate Trp160Cys, 91G>T to generate Asp31Tyr, 502C>T to generate Arg168Cys and 516G>C to generate Glu172Asp mutations) were made (GenScript) and cloned into pCMV6 Empty vector with a DDK-tag at C-terminus (OriGene). Get A Quote

摘要

PR homology domain-containing member 12 (PRDM12) belongs to a family of conserved transcription factors implicated in cell fate decisions. Here we show that PRDM12 is a key regulator of sensory neuronal specification in Xenopus. Modeling of human PRDM12 mutations that cause hereditary sensory and autonomic neuropathy (HSAN) revealed remarkable conservation of the mutated residues in evolution. Expression of wild-type human PRDM12 in Xenopus induced the expression of sensory neuronal markers， which was reduced using various human PRDM12 mutants. In Drosophila， we identified Hamlet as the functional PRDM12 homolog that controls nociceptive behavior in sensory neurons. Furthermore， expression analysis of hum... More

关键词

BSA， bovine serum albumin，Brn3d， brain 3d，CGNL1， cyclin L1，ChIP， chromatin immunoprecipitation，DAPI， 4′，6-diamidino-2-phenylindole，DDK， DYKDDDDK epitope，Drgx， dorsal root ganglia homeobox，ECL， enhanced chemiluminescence，En1， engrailed-1，FDR， false discovery rate，FPKM， fragments per kilobase exon，GAPDH， glyceraldehyde 3-phospate dehydrogenase，GEO， gene expression omnibus，GFP， green fluorescent protein，HEK293， human embryonic kidney cell 293，HRP， horseraddish peroxidase，HSAN， hereditary and sensory autonomic neuropathy，Hamlet，Hmx3， H6 family homeobox 3，IL1R1， interleukin 1 receptor type 1，MO， morpholino oligonucleotide，NBT/BCIP， nitro blue tetrazolium / 5-bromo-4-chloro-3-indolyl-phosphate，PBS， phosphate buffered saline，PDB， protein data base，PMID， pubmed identification.，PRDM12，PRDM12， PR homology domain-containing member 12，RA， retinoic acid，RT-qPCR， real-time quantitative polymerase chain reaction，S1PR1， Sphi8ngosine-1-phosphate receptor 1，SET， Su(var)3–9 and ‘Enhancer of zeste’，Sncg， Synuclein Gamma (Breast Cancer-Specific Protein 1)，TRH(DE)， tryrotropin-releasing hormone degrading enzyme，TRHDE，TRHDE， tyrotropin-releasing hormone degrading enzyme，Tlx3， T-cell leukemia homeobox 3，nociception，pCMV6， plasmid cytomegalovirus，sensory neu
Article Link >