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Bacterial Expression System> | … linker [32]. Based on polypeptide sequence, the synthetic gene was codon optimised<br> for E. coli expression and the optimised sequence was synthesised, cloned into<br> the pUC57 vector and sequenced by <b>GenScript</b>. This final … | Get A Quote |
Trypanosoma cruzi is a flagellate protozoan pathogen that causes Chagas disease. Currently there is no preventive treatment and the efficiency of the two drugs available is limited to the acute phase. Therefore, there is an unmet need for innovative tools to block transmission in endemic areas. In this study, we engineered a novel recombinant molecule able to adhere to the T. cruzi surface, termed scFv-10D8, that consists of a single-chain variable fragment (scFv) derived from mAb-10D8 that targets gp35/50. The synthetic gene encoding scFv-10D8 was cloned and fused to a 6×His tag and expressed in a prokaryotic expression system. Total periplasmic or 6xHis tag affinity-purified fractions of scFv-10D8 retained t... More