Products/Services Used | Details | Operation |
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PCR Cloning and Subcloning> | Plasmid vectors expressing the desired proteins in the Co-IP experiments are listed below: pCMVHA-E2F5 (human, Addgene plasmid #24213), HA-p73α-pcDNA3 (human, Addgene plasmid #22102), pLVDest-GFP-GemC1 (mouse, as previously described in Arbi et al., 2016) and the corresponding mutants mGemC1Δcc (Δ86-137aa), mGemC1Δct (Δ300-339aa),mGemC1ΔΔ (Δ86-137;300-339); pcDNA3.1+/C-(K)DYK-TFDP1 (human, GenScript #OHu23102) and pEGFP-mP73. Briefly, to generate pEGFP-mP73, the cloning strategy was the following: mp73 ORF from pcDNA3.1 (GenScript #OMu22724) was cloned into the XhoI and AgeI restriction sites of the pEGFP-N1 vector (GenBank #U55762). | Get A Quote |
A distinct combination of transcription factors elicits the acquisition of a specific fate and the initiation of a differentiation program. Multiciliated cells (MCCs) are a specialized type of epithelial cells that possess dozens of motile cilia on their apical surface. Defects in cilia function have been associated with ciliopathies that affect many organs, including brain and airway epithelium. Here we show that the geminin coiled-coil domain-containing protein 1 GemC1 (also known as Lynkeas) regulates the transcriptional activation of p73, a transcription factor central to multiciliogenesis. Moreover, we show that GemC1 acts in a trimeric complex with transcription factor E2F5 and tumor protein p73 (official... More