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Proteins, Expression, Isolation and Analysis> | … PBS plus 0.3% Triton X-100 and incubated overnight at 4oC with anti-N-terminal mouse Dct<br> primary antibody at a dilution of 1:800 (custom antibody raised in rabbit, <b>GenScript</b>). After washing<br> in PBS Accepted Article Page 8. This article is protected by copyright … | Get A Quote |
Primary leptomeningeal melanocytic neoplasms represent a spectrum of rare tumors originating from melanocytes of the leptomeninges, which are the inner two membranes that protect the central nervous system. Like other non-epithelial melanocytic lesions, they bear frequent oncogenic mutations in the heterotrimeric G protein alpha subunits, GNAQ or GNA11. In this study, we used Plp1-creERT to force the expression of oncogenic GNAQQ209L in the multipotent neural crest cells of the ventro-medial developmental pathway, beginning prior to melanocyte cell differentiation. We found that this produces leptomeningeal melanocytic neoplasms, including cranial melanocytomas, spinal melanocytomas, and spinal melanomas, in ad... More