Products/Services Used | Details | Operation |
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Gene Synthesis> | Gene synthesis (GenScript) was used to generate a C-terminal triple FLAG-tagged variant (DYKDHDGDYKDHDIDYKDDDDK, separated from the Lsr2 sequence by a triple glycine linker) bearing the same upstream and downstream sequences as the native complementation construct. | Get A Quote |
Lsr2 is a nucleoid-associated protein conserved throughout the actinobacteria, including the antibiotic-producing species encode paralogous Lsr2 proteins (Lsr2 and Lsr2-like, or LsrL), and we show here that of the two, Lsr2 has greater functional significance. We found that Lsr2 binds AT-rich sequences throughout the chromosome, and broadly represses gene expression. Strikingly, specialized metabolic clusters were over-represented amongst its targets, and the cryptic nature of many of these clusters appears to stem from Lsr2-mediated repression. Manipulating Lsr2 activity in model species and uncharacterized isolates resulted in the production of new metabolites not seen in wild type strains. Our... More