Products/Services Used | Details | Operation |
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Recombinant Antibody Services> | Anti-RAS monobody NS1, referred in this paper as “iB(RAS)” was synthetized by GenScript, using amino acid sequence from 5E95 Protein Data Bank entry for reverse translation53. | Get A Quote |
Techniques of protein regulation, such as conditional gene expression, RNA interference, knock-in and knock-out, lack sufficient spatiotemporal accuracy, while optogenetic tools suffer from non-physiological response due to overexpression artifacts. Here we present a near-infrared light-activatable optogenetic system, which combines the specificity and orthogonality of intrabodies with the spatiotemporal precision of optogenetics. We engineer optically-controlled intrabodies to regulate genomically expressed protein targets and validate the possibility to further multiplex protein regulation via dual-wavelength optogenetic control. We apply this system to regulate cytoskeletal and enzymatic functions of two non... More