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Plasmid DNA Preparation> | … The N-terminal FLAG-tagged EcoMRAP2 reported recently by us (Rao et al, 2019), and N-terminal myc-tagged EcoMC1R, were commercially synthesized and subcloned into pcDNA31 by GenScript (Piscataway, NJ, USA) to generate the plasmid used for transfection 22 … | Get A Quote |
Melanocortin-1 receptor (MC1R) has important roles in regulating pigmentation and inflammation. Melanocortin receptor accessory protein 2 (MRAP2) modulates trafficking, ligand binding, and signaling of mammalian melanocortin receptors. However, the effect of MRAP2 on fish MC1R has not been extensively studied. Herein, we cloned the orange-spotted grouper (Epinephelus coioides) mc1r, which had a 972 bp open reading frame encoding a putative protein of 323 amino acids. Grouper mc1r was mainly expressed in the brain, skin, testis, spleen, head kidney, and kidney. EcoMC1R showed high constitutive activities in both Gs-cAMP and ERK1/2 pathways, which could be differentially modulated by grouper MRAP2 (EcoMRAP2). T... More