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Plasmid DNA Preparation> | … The FN short repeats encoding either 3 type-I repeats ( 1–3 FNI) or 2 type-II repeats ( 1–2 FNII) were synthesized by GenScript (Hong Kong) with C-terminal HA and hexahistidine (His) tags, and were subcloned into the pcDNA3 plasmid via the BamHI/EcoRI sites … | Get A Quote |
Heat shock protein 90 (HSP90) is an evolutionarily conserved chaperone protein that controls the function and stability of a wide range of cellular client proteins. Fibronectin (FN) is an extracellular client protein of HSP90, and exogenous HSP90 or inhibitors of HSP90 alter the morphology of the extracellular matrix. Here, we further characterized the HSP90 and FN interaction. FN bound to the M domain of HSP90 and interacted with both the open and closed HSP90 conformations; and the interaction was reduced in the presence of sodium molybdate. HSP90 interacted with the N-terminal regions of FN, which are known to be important for matrix assembly. The highest affinity interaction was with the 30-kDa (heparin-bin... More