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Custom Vector Construction> | … NLS: nuclear localization signal FKBP conserved domains were forecasted by NCBI, SMART (http://smartembl-heidelbergde/), and PSORT (https://wwwgenscriptcom/psorthtml) (B) Schematic model of construction for FKBP gene replacement vectors … | Get A Quote |
FK506 binding proteins (FKBPs) participate in regulation of diverse biological processes. However, the role of these proteins in insect-pathogenic fungi is far from well understood. To investigate the functions of FKBPs in Beauveria bassiana, a widely used entomopathogenic fungus for control of insect pests, we identify three putative FKBP genes, Bbfkbp12, Bbfkbp15, and Bbfkbp50, in the fungus. Gene-disruption experiments show that loss of Bbfkbp12 results in a significant increase of resistance of B. bassiana against the immunosuppressive compounds FK506 and rapamycin, while loss of Bbfkbp50 leads to the resistance to the ergosterol synthesis inhibitor lovastatin. Transcription assays of calcineurin (CaN)- and... More