Products/Services Used | Details | Operation |
---|---|---|
Plasmid DNA Preparation> | To generate SIMx4 probe we used a synthesized plasmid construction containing the cod- ing sequence for aminoacids 32–132 of RNF4 from Rattus norvegicus (GenScript).... Generation of TbSUMO-transfectant cell lines For HisHA-TbSUMO strain, a HisHA-TbSUMO construct was synthesized (GenScript), con- taining the coding sequence for an 8His (24bp)-HA (27bp) tag, 249bp of the 5’end of TbSUMO open reading frame (ORF; Tb927. | Get A Quote |
SUMOylation is a post-translational modification conserved in eukaryotic organisms that involves the covalent attachment of the small ubiquitin-like protein SUMO to internal lysine residues in target proteins. This tag usually alters the interaction surface of the modified protein and can be translated into changes in its biological activity, stability or subcellular localization, among other possible outputs. SUMO can be attached as a single moiety or as SUMO polymers in case there are internal acceptor sites in SUMO itself. These chains have been shown to be important for proteasomal degradation as well as for the formation of subnuclear structures such as the synaptonemal complex in Saccharomyces cerevisiae ... More