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Proteins, Expression, Isolation and Analysis> | After concentration measurement, 30 µg of cell lysates was resolved on 4–20% ExpressPluS PAGE Gel (GenScript, Piscataway, New Jersey, USA).... The proteins were then transferred onto PVDF Transfer Membrane (Thermo Scientific, Rockford, Illinois, USA) using eBlot Protein Transfer device (GenScript, Piscataway, New Jersey, USA). | Get A Quote |
Cancer cells often accumulate spontaneous and treatment-induced DNA damage i.e. potentially lethal DNA double strand breaks (DSBs). Targeting DSB repair mechanisms with specific inhibitors could potentially sensitize cancer cells to the toxic effect of DSBs. Current treatment for glioblastoma includes tumor resection followed by radiotherapy and/or temozolomide (TMZ) - an alkylating agent inducing DNA damage. We hypothesize that combination of PARP inhibitor (PARPi) with TMZ in glioblastoma cells displaying downregulation of DSB repair genes could trigger synthetic lethality. In our study, we observed that PARP inhibitor (BMN673) was able to specifically sensitize DNA ligase 4 (LIG4)-deprived glioblastoma cells... More