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Monoclonal Antibody Services> | Generation of hybridoma and production of antibodies Monoclonal antibodies against CVB3 (Nancy) 2Apro and 3Cpro pro- teases were produced through the immunization of Wistar rats with the purified proteases (GenScript, Hong Kong).... Hybridomas were generated by electrofusion and the positive clones were positively selected for by the detection of the proteases and negatively selected for their re- cognition of the His-tag by ELISA (Genscript). | Get A Quote |
Enteroviruses (EVs), such as the Coxsackie B-viruses (CVBs), are common human pathogens, which can cause severe diseases including meningitis, myocarditis and neonatal sepsis. EVs encode two proteases (2Apro and 3Cpro), which perform the proteolytic cleavage of the CVB polyprotein and also cleave host cell proteins to facilitate viral replication. The 2Apro cause direct damage to the infected heart and tools to investigate 2Apro and 3Cpro expression may contribute new knowledge on virus-induced pathologies. Here, we developed new antibodies to CVB-encoded 2Apro and 3Cpro; Two monoclonal 2Apro antibodies and one 3Cpro antibody were produced. Using cells infected with selected viruses belonging to the EV A, B and... More