Products/Services Used | Details | Operation |
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Custom Vector Construction> | 1) was synthetically generated (GenScript) and cloned into pET-28b expression vector as a N-terminally His6- tagged protein with an internal thrombin cleavage site for removal of the His6 tag after purification. | Get A Quote |
The rapid spread of the recent Zika virus (ZIKV) epidemic across various countries in the American continent poses a major health hazard for the unborn fetuses of pregnant women. To date, there is no effective medical intervention. The nonstructural protein 5 of Zika virus (ZIKV-NS5) is critical for ZIKV replication through the 5'-RNA capping and RNA polymerase activities present in its N-terminal methyltransferase (MTase) and C-terminal RNA-dependent RNA polymerase (RdRp) domains, respectively. The crystal structure of the full-length ZIKV-NS5 protein has been determined at 3.05 Å resolution from a crystal belonging to space group P21212 and containing two protein molecules in the asymmetric unit. The struc... More