| Products/Services Used | Details | Operation |
|---|---|---|
| Custom Vector Construction | 2-IMP1 PABPC1-GFP DCP1a-GFP pHcRed1-G3BP Software and Algorithms ZEN 2012 RStudio ATCC CCL-2 Eurofins Genomics N/A LGC Biosearch LGC Biosearch LGC Biosearch Clontech This manuscript This manuscript Genscript This manuscript This manuscript This manuscript This manuscript This manuscript Carl Zeiss Microscopy R Foundation Cat#VSMF-2002-5 Cat#VSMF-2003-5 Cat#SMF-2026-1 N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A LEAD CONTACT AND MATERIALS AVAILABILITY Further information and requests for resources and reagents should be directed to and will be fulfilled by the Lead Contact, Finn Cilius Nielsen (finn....1+N-EGFP (Genscript) and pmCherry-C1 (Clontech) vectors, inserting a 25 amino acid flexible linker composed of 5x GlyGlyGlyGlySer between the fluorescent tag and YBX1 to reduce aggregation. | Get A Quote |
Small cytoplasmic mRNP granules are implicated in mRNA transport, translational control, and decay. Using super-resolution microscopy and fluorescence correlation spectroscopy, we analyzed the molecular composition and dynamics of single cytoplasmic YBX1_IMP1 mRNP granules in live cells. Granules appeared elongated and branched, with patches of IMP1 and YBX1 distributed along mRNA, reflecting the attachment of the two RNA-binding proteins in cis. Particles form at the nuclear pore and do not associate with translating ribosomes, so the mRNP is a repository for mRNAs awaiting translation. In agreement with the average number of mRNA-binding sites derived from crosslinked immunoprecipitation (CLIP) analyses, indi... More
