Products/Services Used | Details | Operation |
---|---|---|
Peptide Synthesis> | To form the CBC, the pellet of SF9 insect cells infected with baculovirus expressing HIS-tagged CBP80(Δ2–23) (Genscript) was resuspended in 40 mL of Q column buffer A (200 mM NaCl, 50 mM HEPES at pH 7....8 mg/mL CBC, 5 µL of 20 µg/mL PGC-1β peptide spanning amino acids 994– 1023 (Ac-SEEALPASGKSKYEAMDFDSLLKEAQQSLH, where p-iodo-tyrosine [L-3-I-Tyr] was used in place of Y1006 for possible phasing applications; GenScript) (Xie et al. | Get A Quote |
Although peroxisome proliferator-activated receptor-γ (PPARγ) coactivator 1α (PGC-1α) is a well-established transcriptional coactivator for the metabolic adaptation of mammalian cells to diverse physiological stresses, the molecular mechanism by which it functions is incompletely understood. Here we used in vitro binding assays, X-ray crystallography, and immunoprecipitations of mouse myoblast cell lysates to define a previously unknown cap-binding protein 80 (CBP80)-binding motif (CBM) in the C terminus of PGC-1α. We show that the CBM, which consists of a nine-amino-acid α helix, is critical for the association of PGC-1α with CBP80 at the 5' cap of target transcripts. Results from RNA sequencing demonst... More