| Products/Services Used | Details | Operation |
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| Catalog Antibody | Antibodies Antibody dilutions used for immunoblotting were as follows: 1:10,000 of mouse anti- β-catenin (BD Transduction Laboratories, San Jose, CA, USA), 1:5,000 of mouse anti-GFP (Roche, Indianapolis, IN, USA), 1:3000 rabbit anti-flag (Genscript, Piscaraway, NJ, USA).... Antibodies used for immunofluorescence were diluted at 1:1000; rabbit anti-EEA1 (Abcam, Cambridge, MA, USA), mouse anti-transferrin receptor (TfR) (Abcam, Cambridge, MA, USA), mouse anti-HA (Santa Cruz, Dallas, TX, USA), mouse anti- flag (Genscript, Piscataway, NJ, USA), mouse anti-Rab9 (Affinity Bioreagents, Golden, CO, USA), mouse anti-Rab7 (Abcam, Cambridge, MA, USA), mouse anti- Rab11 (Abcam, Cambridge, MA, USA), rabbit anti-Rab4 (Thermo Scientific, Rockford, IL, USA), and Surface-CLIP 547 or 488 (New England Biolabs, Ipswich, MA, USA). | Get A Quote |
Cu ion (Cu) entry into human cells is mediated by CTR1 (also known as SLC31A1), the high-affinity Cu transporter. When extracellular Cu is raised, the cell is protected against excess accumulation by rapid internalization of the transporter. When Cu is lowered, the transporter returns to the membrane. We show in HEK293 cells overexpressing CTR1 that expression of either the C-terminal domain of AP180 (also known as SNAP91), a clathrin-coat assembly protein that sequesters clathrin, or a dominant-negative mutant of dynamin, decreases Cu-induced endocytosis of CTR1, as does a dynamin inhibitor and clathrin knockdown using siRNA. Utilizing imaging, siRNA techniques and a new high-throughput assay for endocytosis e... More
