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Gene Synthesis> | [8] The following C-terminally His6-tagged alcohol dehydrogenase genes were synthesised and sub cloned into pET21b by GenScript: (-)-trans-isopiperitenol dehydrogenase from Mentha piperita (IPDH; UNIPROT: Q5C919),[7] (1S,5R)-carveol dehydrogenase from Rhodococcus erythropolis (CDH; UNIPROT: Q9RA05),[21] (R)-specific alcohol dehydrogenase from Lactobacillus kefir (LkADH; UNIPROT: Q6WVP7)[36] and secondary alcohol dehydrogenase from Rhodococcus ruber DSM 44541 (Rr-ADH; UNIPROT: Q8KLT9). | Get A Quote |
The monoterpenoid lactone derivative (+)-dihydrocarvide ((+)-DHCD) can be polymerised to form shape-memory polymers. Synthetic biology routes from simple, inexpensive carbon sources are an attractive, alternative route over chemical synthesis from (R)-carvone. We have demonstrated a proof-of-principle in vivo approach for the complete biosynthesis of (+)-DHCD from glucose in Escherichia coli (6.6 mg L-1 ). The pathway is based on the Mentha spicata route to (R)-carvone, with the addition of an 'ene'-reductase and Baeyer-Villiger cyclohexanone monooxygenase. Co-expression with a limonene synthesis pathway enzyme enables complete biocatalytic production within one microbial chassis. (+)-DHCD was successfully ... More