Products/Services Used | Details | Operation |
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Proteins, Expression, Isolation and Analysis> | The mAbs were purified from the culture supernatants using Protein G agarose beads (Genscript) and further purified on a Superdex HiLoad 16/60 75 or 200 pg column (GE Healthcare). | Get A Quote |
Because monoclonal antibodies (mAbs) have exceptional specificity and favorable pharmacology, substantial efforts have been made to functionalize them, either with potent cytotoxins, biologics, radionuclides, or fluorescent groups for therapeutic benefit and/or use as theranostic agents. To exploit our recently discovered meditope-Fab interaction as an alternative means to efficiently functionalize mAbs, we used insights from the structure to enhance the affinity and lifetime of the interaction by four orders of magnitude. To further extend the lifetime of the complex, we created a mechanical bond by incorporating an azide on the meditope, threading the azide through the Fab, and using click chemistry to add a ... More