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Flexible IgE epitope-containing domains of Phl p 5 cause high allergenic activity.

J Allergy Clin Immunol. 2017;

Göbl C, Focke-Tejkl M, Najafi N, Schrank E, Madl T, Kosol S, Madritsch C, Dorofeeva Y, Flicker S, Thalhamer J, Valenta R, Zangger K, Tjandra N.

Products/Services Used	Details	Operation
Custom Vector Construction	DNA encoding two N-terminal (NTD, tNTD) fragments with N-terminal hexahistidine tags and one C-terminal fragment (CTD) of Phl p 5a containing a C-terminal hexahistidine tag were synthesized (GenScript, Piscataway, NJ, USA), cloned into expression vector pET17b, and expressed in E coli BL21 (DE3).	Get A Quote