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Proteins, Expression, Isolation and Analysis> | The resuspended proteins were boiled at 908C for 10 min and an aliquot was resolved on 8% SDS-PAGE or precast ExpressPlus 4–15% gradient gel (GenScript #M41212) following the manufacturer’s SDS-PAGE protocol. | Get A Quote |
Resistance to azole drugs, the major clinical antifungal compounds, is most commonly due to gain-of-function (GOF) substitution mutations in a gene called PDR1 in the fungal pathogen Candida glabrata. PDR1 encodes a zinc cluster-containing transcription factor. GOF forms of Pdr1 drive high level expression of downstream target gene expression with accompanying azole resistance. PDR1 has two homologous genes in Saccharomyces cerevisiae, called ScPDR1 and ScPDR3. This study provides evidence that the PDR1 gene in C. glabrata represents a blend of the properties found in the two S. cerevisiae genes. We demonstrated that GOF Pdr1 derivatives are overproduced at the protein level and less stable than the wild-type p... More