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Gene Synthesis> | The C-terminal FLAG-tag CyanoP strains (CyanoP-FLAG/WT, CP47, D1 and D2, respectively) were prepared by replacing the sll1418 gene using the synthetic DNA construct (Genscript) containing the upstream region (292 bp), the sll1418 plus 3FLAG sequence (Sigma), a zeocin resistance cassette and the downstream region (269 bp) as depicted in Supplementary Fig. | Get A Quote |
Although the PSII complex is highly conserved in cyanobacteria and chloroplasts, the PsbU and PsbV subunits stabilizing the oxygen-evolving Mn4CaO5 cluster in cyanobacteria are absent in chloroplasts and have been replaced by the PsbP and PsbQ subunits. There is, however, a distant cyanobacterial homolog of PsbP, termed CyanoP, of unknown function. Here we show that CyanoP plays a role in the early stages of PSII biogenesis in Synechocystis sp. PCC 6803. CyanoP is present in the PSII reaction center assembly complex (RCII) lacking both the CP47 and CP43 modules and binds to the smaller D2 module. A small amount of larger PSII core complexes co-purifying with FLAG-tagged CyanoP indicates that CyanoP can accompan... More