| Products/Services Used | Details | Operation |
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| Proteins, Expression, Isolation and Analysis | biotinylated Protein L (GenScript, Piscataway, NJ)-coated Streptavidin MyOne T1 Dynabeads (Invitrogen) were added to the supernatant, and the mixture was incubated at 4 °C with end-over-end rotation overnight. | Get A Quote |
The rapid elimination of dying neurons and nonfunctional synapses in the brain is carried out by microglia, the resident myeloid cells of the brain. Here we show that microglia clearance activity in the adult brain is regionally regulated and depends on the rate of neuronal attrition. Cerebellar, but not striatal or cortical, microglia exhibited high levels of basal clearance activity, which correlated with an elevated degree of cerebellar neuronal attrition. Exposing forebrain microglia to apoptotic cells activated gene-expression programs supporting clearance activity. We provide evidence that the polycomb repressive complex 2 (PRC2) epigenetically restricts the expression of genes that support clearance acti... More
