Products/Services Used | Details | Operation |
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Gene Synthesis> | Methanosarcina barkeri PylRS cDNA (GenScript).... Synthetic DNA encoding OaAEP1b (GenBank accession num- ber KR259377 for OaAEP1 with substitutions 9A ! G and 1112A ! T to produce OaAEP1b) and the cyclotide precursor Oak1 (AF393825) with 5 BamHI and 3 SalI restriction sites (GenScript). | Get A Quote |
Immunoconjugates are essential tools in diagnostics for the detection and quantification of proteins and in cell biology for the characterization of different cell populations as well as for tracking intracellular pathways. In recent years, antibody-drug conjugates (ADCs) have emerged as promising therapeutics to treat cancer and have moved into the focus of interest of the pharmaceutical industry. Here we describe a conjugation method for the generation of antibody conjugates that relies on the formation of a spontaneous isopeptide bond between two peptide tags referred to as SpyTag and KTag. This reaction is catalyzed by SpyLigase, an engineered cell surface protein obtained from Streptococcus pyogenes. We de... More