Products/Services Used | Details | Operation |
---|---|---|
Custom Vector Construction> | … synthesis. S100A9 was amplified via PCR and cloned into the pCI-neo vector with a 3′ HA tag. Sequence verification of the resulting plasmid, pCI-S100A9-HA, was conducted by Genscript Biotechnology Co., Ltd. (Nanjing, China) … | Get A Quote |
Porcine reproductive and respiratory syndrome virus (PRRSV) has caused huge economic losses to the pig industry worldwide over the last 30 years, yet the associated viral-host interactions remain poorly understood. S100A9 is a damage-associated molecular pattern of the S100 protein family. Here, we found that PRRSV infection stimulated S100A9 expression in porcine alveolar macrophages (PAMs) and Marc-145 cells. S100A9 inhibited PRRSV replication via cellular Ca2+ dependent manner. The viral nucleocapsid (N) protein co-localized with S100A9 in the cytoplasm, and directly interacted at amino acid 78 of S100A9 and amino acids 36-37 of N protein. Moreover, we also found that the mutant S100A9 (E78Q) protein exhibit... More