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Gene Synthesis> | … were used to clone the flag tagged Vpr genes of HIV-1 Ug and HIV-1 Cy into pCDNA3.1/hygro (+) (HindIII and XhoI, ThermoFisher) while the flag tagged Vpr genes of SIVgor, SIVcpz, and SIVagm 9063–2 were synthesized into pcDNA3.1/Hygro (+) by GenScript (Piscataway, NJ … | Get A Quote |
Unlike HIV-1, HIV-2 and some SIV strains replicate at high dNTP concentrations even in macrophages due to their accessory proteins, Vpx or Vpr, that target SAMHD1 dNTPase for proteasomal degradation. We previously reported that HIV-1 reverse transcriptase (RT) efficiently synthesizes DNA even at low dNTP concentrations because HIV-1 RT displays faster pre-steady state kpol values than SAMHD1 counteracting lentiviral RTs. Here, since the kpol step consists of two sequential sub-steps post dNTP binding, conformational change and chemistry, we investigated which of the two sub-steps RTs from SAMHD1 non-counteracting viruses accelerate in order to complete reverse transcription in the limited dNTP pools found in ma... More