Products/Services Used | Details | Operation |
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Peptide Synthesis> | … Kinetic reaction mixtures (10μL final volume) included 50mM Tris-HCl pH 8.0, 150mM NaCl, 2mM CaCl 2 , 10mM rhodamine-QALPETGG (GenScript) peptide, and varying concentrations of GGGGDYK-biotin peptide (GenScript) ranging from 0–2.5mM final concentration … | Get A Quote |
The Sortase family of transpeptidases are found in numerous gram-positive bacteria and involved in divergent physiological processes including anchoring of surface proteins to the cell wall as well as pili assembly. As essential proteins, sortase enzymes have been the focus of considerable interest for the development of novel anti-microbials, however, more recently their function as unique transpeptidases has been exploited for the synthesis of novel bio-conjugates. Yet, for synthetic purposes, SrtA-mediated conjugation suffers from the enzyme's inherently poor catalytic efficiency. Therefore, to identify SrtA variants with improved catalytic efficiency, we used directed evolution to select a catalytically enh... More