Products/Services Used | Details | Operation |
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Gene Synthesis> | … Ki67 (Santa Cruz); Flag-tagged Ab (Sigma); His-tagged Ab (GenScript Corporation); GAPDH (Kangchen, Shanghai). λ-PPase (New England Biolabs). SiRNA and lentiviral production. SiRNA-PAK1 and control siRNA were purchased from Nanjing genepharma Company … | Get A Quote |
To date, microrchidia (MORC) family CW-type zinc-finger 2 (MORC2), has been found to be involved in p21-activated kinase1 (PAK1) pathway to maintain genomic integrity. Here, we explore its novel role in cancer. We demonstrate that PAK1-mediated MORC2 phosphorylation promotes cell cycle progression, defective phosphorylation of MORC2-S677A results in attenuated cell proliferation and tumorigenicity of gastric cancer cells, which is significantly enhanced in overexpression of phospho-mimic MORC2-S677E form, suggesting the importance of MORC2 phosphorylation in tumorigenesis. More importantly, phosphorylation of MORC2 correlates positively with PAK1 expression in clinical gastric cancer. Furthermore, high expressi... More