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Custom Vector Construction> | … The nucleotide sequence was optimized for expression in Ecoli by GenScript (https://www genscriptcom) LytU-SH3b optimized construct was synthesized and sub-cloned in the NcoI and XhoI sites of vector pET28a by Biomatik Company (Biomatik, Ontario, Canada) … | Get A Quote |
Methicillin-resistant Staphylococcus aureus (MRSA) is a challenging infectious agent worldwide. The ever growing antibiotic resistance has made the researchers to look for new anti-staphylococcal agents. Autolysins are staphylococcal enzymes that lyse bacterial cell wall for cell division. Autolysins can be used as novel enzybiotics (enzymes have antibiotic effects) for staphylococcal infections. LytU is a newly explored autolysin. SH3b is a potent cell wall binding domain that can be fused to lytic enzymes to increase their activity. The aim of this study was to design a novel and efficient fusion enzybiotic that could lyse staphylococcal cell wall peptidoglycan by disrupting the bacteria. LytU-SH3b fusion con... More