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Biomolecular engineering of antidehydroepiandrosterone antibodies: a new perspective in cancer diagnosis and treatment using single-chain antibody variable fragment.

Nanomedicine (Lond). 2019;

Fogaça RL,, Alvarenga LM,,, Woiski TD, Becker-Finco A, Teixeira KN, Silva SK,, de Moraes RN, Noronha L,, Noiray M, de Figueiredo BC, Billiald P,, Moura J,,.

Products/Services Used	Details	Operation
Gene Synthesis	… Synthetic genes encoding 5B7 IGHV A or B fused to the IGKV (immunoglobulin κ variable region) counterpart via a (Gly 4 Ser) 3 peptide linker and a C-terminal His 6 tag were designed with the codon sequence optimized at Genscript USA Inc …	Get A Quote

摘要

To develop a monoclonal antibody against dehydroepiandrosterone (DHEA) and miniaturize it, generating a single-chain antibody variable fragment (scFv) against DHEA as an adrenocortical carcinoma (ACC) marker.,DHEA conjugated to keyhole limpet hemocyanin was used as an immunogen to obtain anti-DHEA hybridomas. Variable fragments were cloned from hybridoma 5B7 total RNA, and used to detect DHEA in normal adrenal tissue and ACC cells.,IgM monoclonal antibody was highly specific, and the recombinant scFv preserved parental antibody characteristics, allowing tissue localization of DHEA.,Undefined small lesions are challenges for clinicians and impact clinical adrenocortical tumor management. Generating an anti-DHEA ... More