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PFM-Like Enzymes Are a Novel Family of Subclass B2 Metallo-β-Lactamases from Pseudomonas synxantha Belonging to the Pseudomonas fluorescens Complex.

Antimicrob Agents Chemother. 2020; 
Poirel L,,, Palmieri M,, Brilhante M, Masseron A, Perreten V, Nordmann P,,,.
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Proteins, Expression, Isolation and Analysis … 139 The protein concentrations were measured using Bradford reagent (Sigma-Aldrich, 140 Buchs, Switzerland) and the purity of the enzyme was estimated by SDS-PAGE analysis 141 (GenScript, NJ, USA) The purification of PFM-1 was estimated to be >95% with a single 142 … Get A Quote

摘要

A carbapenem-resistant Pseudomonas synxantha isolate recovered from chicken meat produced the novel carbapenemase PFM-1. That subclass B2 metallo-β-lactamase shared 71% amino acid identity with β-lactamase Sfh-1 from Serratia fonticola The bla PFM-1 gene was chromosomally located and likely acquired. Variants of PFM-1 sharing 90% to 92% amino acid identity were identified in bacterial species belonging to the Pseudomonas fluorescens complex, including Pseudomonas libanensis (PFM-2) and Pseudomonas fluorescens (PFM-3), highlighting that these species constitute reservoirs of PFM-like encoding genes.,Copyright © 2020 American Society for Microbiology.

关键词

PFM-1; Pseudomonas fluorescens ; metallo-beta-lactamase