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DNA Sequencing> | … PCR fragments were cloned into the EcoRI and XhoI sites of pCMV-Tag2B (gift from Paul Roche, NIH), which was modified to contain a 3xFLAG tag instead of a single FLAG tag DNA sequences were confirmed by Sanger sequencing (GenScript) Page 9 T Monteiro-Brás et al … | Get A Quote |
Chlamydia trachomatis is an obligate intracellular pathogen that replicates inside a parasitic vacuole called the inclusion. The nascent inclusion is derived from the host plasma membrane and serves as a platform from which Chlamydia controls interactions with the host microenvironment. To survive inside the host cell, Chlamydia scavenges for nutrients and lipids by recruiting and/or fusing with various cellular compartments. The mechanisms by which these events occur are poorly understood but require host proteins such as the SNARE proteins (SNAP (Soluble N-ethylmaleimide-sensitive factor attachment protein) Receptor). Here, we show that SNAP-23 and Syntaxin 4, two plasma membrane SNAREs, are recruited to the ... More