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Gene Synthesis> | … The optimized gene was synthesized and cloned in pMAL-p5x vector, and the expression construct was received from Genscript USA Construct Designs of CD151 Different constructs of CD151 were designed to facilitate the recombinant expression in E coli (Figs … | Get A Quote |
CD151 is an abundantly expressed eukaryotic transmembrane protein on the cell surface. It is involved in cell adhesion, angiogenesis and signal transduction as well in disease conditions such as cancer and viral infections. However, the molecular mechanism of CD151 activation is poorly understood due to the lack of structural information. By considering the difficulties in expressing the membrane protein in E. coli, herein we introduce the strategic design for the effective expression of recombinant CD151 protein in E. coli with high yield, that would aid for the structural studies. CD151 having four transmembrane domain (TMD's) along with small and a large extracellular loop (LEL) is constructed in parts to en... More