Products/Services Used | Details | Operation |
---|---|---|
Custom Vector Construction> | The gD-NSP gene shown in Figure 8(b) incorporating the addition of C-terminal His-tag residues SGGSGGHHHHHH was cloned into the bacterial expression vector pET17b using the restriction sites Nde1 and Xhol (5 and 3, respectively). Cloning, sequence verification, and quality analysis of the resulting plasmid gD-NSP_pET17A were performed by GenScript (Canton, MA). | Get A Quote |
Nanoparticles are playing an increasingly powerful role in vaccine development. Here, we report the repurposing of nonstructural proteins 10 and 11 (hereafter NSP10) from the replicase polyprotein 1a (pp1a) of the human SARS coronavirus (severe acute respiratory syndrome) as a novel self-assembling platform for bioengineered nanoparticles for a variety of applications including vaccines. NSP10 represents a 152 amino acid, 17 kD zinc finger transcription/regulatory protein which self-assembles to form a spherical 84 Å diameter nanoparticle with dodecahedral trigonal 32 point symmetry. As a self-assembling nanoparticle, NSP10 possesses numerous advantages in vaccine development and antigen display, including... More