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ORF cDNA Clones/MolecularCloud> | For antibody, mouse anti-Clorf109 monoclonal antibody was purchased from Genscript company (Nanjing, China.WB 1:1000 dilution), rabbit anti-eGFP polyclonal antibody(Abcam, author/funder. All rights reserved. No reuse allowed without permission. bioRxiv preprint doi: https://doi.org/10.1101/625749. The copyright holder for this preprint (which was not peer-reviewed) is the WB 1:1000 dilution and IP:2 μg/ml, ab290), rabbit anti-PARP1 monoclonal antibody(Cell Signaling technology, WB 1:1000, Lot#9532), rabbit anti-phospho-Histone H2A.X(Ser139)(20E3) monclonal antibody(Cell Signaling technology, WB 1:1000 dilution, Lot#9718), rabbit anti-CDK1 polyclonal antibody(Proteintech, WB 1:2000 dilution, Catalog No:199532-1-AP), rabbit anti-CyclinB1 polyclonal antibody (Proteintech,WB 1:1000 dilution,Catalog No:55004-1-AP), rabbit anti-CyclinA2 polyclonal antibody(Proteintech, WB 1:1000 dilution, Catalog No:18202-1-AP), mouse anti-DHX9 polyclonal antibody (Abcam, WB 1:1000 dilution, ab26271), mouse anti-phospho-CDK1-T14 monclonal antibody(ABclonal, WB 1:1000 dilution, Catalog No:AP0015), rabbit anti-RNASEH1 polyclonal antibody(ABclonal, WB 1:1000 dilution, Catalog No:A9116), anti-GAPDH(ABclonal, WB 1:50000 dilution,AC033), mouse anti-S9.6 monclonal antibody (EMD millipore, IF 1:50 dilution, Lot#301193 ), mouse Flag-Tag monclonal antibody(Thermo Fisher,WB 1:1000 dilution, Catalog No: MA1-91878), rabbit anti-Histone H3ac(pan-acetyl) polyclonal antibody(Active Motif, WB 0.2μg/ml, Catalog No: 61637,61638 ), rabbit anti-Histone H3 polyclonal antibody(Proteintech, WB 1:1000, Catalog No:17168-1-AP), rabbit anti-NPM1(N-term) polyclonal antibody(Absin, WB 1:1000, abs106184) | Get A Quote |
As a function unknown gene, C1orf109 is lower expression in various cells. Here, we reported that C1orf109L, the longest variant of C1orf109, which interacted with R-loop-regulating proteins to trigger R-loop, a three-stranded nucleic acid structure frequently mediated genome instability, accumulation. C1orf109L induce chronic DNA damage to promote P21 upregulation and strongly inhibits cell growth in vitro and in vivo by arresting the cell cycle in the G2 phase. With camptothecin (CPT), an R-loop activator, treatment, C1orf109L further triggers R-loop accumulation-induced DNA damage and promotes cell death by activating cell-death pathway. Furthermore, CPT treatment increases C1orf109L ubiquitination and t... More