Products/Services Used | Details | Operation |
---|---|---|
Gene Synthesis> | Additionally, a vector expressing C-terminus His-tagged Rgs12 (Rgs12-His) was generated by 2 subcloning the Rgs12 cDNA into the pcDNA3.1(+)-c-His vector (Genscript, Piscataway, NJ, 3 USA). | Get A Quote |
The Regulator of G-protein Signaling 12 (Rgs12) is important for osteoclast (OC) differentiation, and its deletion in vivo protected mice against pathological bone loss. To characterize its mechanism in osteoclastogenesis, we selectively deleted Rgs12 in OC precursors using the LysM-Cre transgenic line or overexpressed the gene in RAW264.7 cells. Rgs12 deletion led to increased bone mass with decreased OC numbers, whereas its overexpression increased OC number and size. Proteomics analysis of Rgs12-deficient OCs identified an upregulation of antioxidant enzymes under the transcriptional regulation of Nrf2, the master regulator of oxidative stress. We confirmed an increase of Nrf2 activity and impaired productio... More