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Gene Synthesis> | The modified plasmid contained hAFN-H gene flanked between 5' NdeI and BamHI restriction sites, as along with the genes for protein-G and 6×His-tag (GenBank: P19909 GenScript) flanked between BamHI, and 3' XhoI restriction sites in the C-terminus orientation. | Get A Quote |
Salmon fish farmers face remarkable problems in fish rearing and handling due to the spread of disease by infectious pancreatic necrosis virus (IPNV). Therefore, we developed a straightforward and sensitive technique to detect IPNV-based on recombinant human apoferritin heavy chain (hAFN-H) protein nanoparticles. In this study, the 24 subunits of the hAFN-H were genetically modified to express 6×His-tag and protein-G at their C-terminal site using Escherichia coli. We thus achieved a two-step signal amplifying strategy that utilizes a recombinant hAFN-H nanoprobe having a protein-G-binding site that targets the Fc region of monoclonal antibodies and a 6×His-tag that actively interacts with the functionalized ... More