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Molecular Biology Tools> | … com); recombinant human GFPT1 protein and GFPT1 cDNA clone from Sino Biological (Beijing, China; http://jpsinobiologicalcom); and human, mouse, rat, zebra fish, and chicken GFPT1 and GFPT2 cDNA clones from GenScript (Piscataway, NJ; http://wwwgenscriptcom) … | Get A Quote |
Glutamine-fructose-6-phosphate transaminase 1 (GFAT) is the rate-limiting enzyme of the hexosamine pathway that has been implicated in the pathogenesis of diabetic nephropathy. As such, we hypothesized that GFPT1, which encodes for GFAT, may confer genetic susceptibility to this complication among Caucasians. Screening of all known functional regions of GFPT1 revealed six single nucleotide polymorphisms (SNPs) that were located in the promoter, introns, and 3' untranslated region. The approximately 60 kb GFPT1 locus was encompassed in a single conserved haplotype block, and two tagging SNPs were sufficient to capture >90% of the haplotype diversity. Analysis of these SNPs in a case-control study made up of type... More