| Products/Services Used | Details | Operation |
|---|---|---|
| PCR Cloning and Subcloning | 5) with the N-terminal GST-tag was done in the vector pGEX-6P-1, using the cloning site BamHI- XhoI, provided by GenScript (Hong Kong, HK)....1 expression vector containing the full-length human14-3-3e sequence was obtained from GenScript (Hong Kong, HK). | Get A Quote |
Ras-selective lethal small molecule 3 (RSL3), a drug candidate prototype for cancer chemotherapy, triggers ferroptosis by inactivating the glutathione peroxidase glutathione peroxidase 4 (GPx4). Here, we report the purification of the protein indispensable for GPx4 inactivation by RSL3. Mass spectrometric analysis identified 14-3-3 isoforms as candidates, and recombinant human 14-3-3ε confirms the identification. The function of 14-3-3ε is redox-regulated. Moreover, overexpression or silencing of the gene coding for 14-3-3ε consistently controls the inactivation of GPx4 by RSL3. The interaction of GPx4 with a redox-regulated adaptor protein operating in cell signaling further contributes to frame it within r... More
