| Products/Services Used | Details | Operation |
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| Peptide Synthesis | The pKa of the MMPXCXX thiol containing tag sequences (XCXX), Ac-GCRDG-NH¬2 (CRD), Ac-GDCDDG-NH2 (DCDD), Ac-GECEEG-NH2 (ECEE) (Genscript, Piscataway, NJ) was determined as previously described [11]. Briefly, 0.01 M sodium acetate, potassium phosphate, tris base, or sodium tetraborate decahydrate was added to 0.1 M NaCl to create various pH buffers. Aliquots with 0.15 μmol of lyophilized thiol-containing peptides, were dissolved in 1500 μL of pH buffers ranging from pH 4 to pH 12 for a final concentration of 0.1 mM. The absorbance at 240 nm of the thiolates in each aliquot was measured in a NanoDrop 2000c spectrophotometer (NanoDrop Technologies Inc., Wilmington, DE). | Get A Quote |
The development of synthetic hydrogels analogs for the extracellular matrix has proven a useful and important tool to study the role of specific signals on biological outcomes in vitro and to serve as scaffolds for tissue repair. Although the importance of physical properties (e.g. microstructure and stiffness) in the micro and nano scale on cell fate has been widely reported, bulk modulus measurements are typically used to characterize hydrogels. Thus, the physical properties of hydrogels have not been widely tested for their controlled physical properties in the nano and micron scales. In this report, we show that although fast Michael-type addition crosslinked hydrogels appear uniform by bulk modulus read... More
