| Products/Services Used | Details | Operation |
|---|---|---|
| Recombinant Proteins | 4-Chlorophenyl methyl sulfone was purchased from Alfa Aesar (Ward Hill, MA). N-(3-Aminopropyl)methacrylamide hydrochloride, >98% (APMA) was purchased from Polysciences, Inc. (Warrington, PA). 1,2-Bis(2-(4,5-dihydro-1H-imidazol-2-yl)propan-2-yl)diazene dihydrochloride (VA-044) was purchased from Toronto Research Chemicals (Toronto, ON). CGRGDS >95% was purchased from GenScript (Piscataway, NJ). Calcein AM fluorescent dye was purchased from Corning, New York, USA. PrestoBlue™ cell viability reagent, Hoechst 33342, HyClone™ calf bovine serum (CBS) and DMEM/F12 1:1 were purchased from Thermo Fisher Scientific (Whitby, ON). Methylcellulose (MC; METOLOSE® SM-4000) was purchased from Shin-Etsu Corp (Tokyo, Japan). Solvents were reagent grade and obtained from Caledon Laboratories (Georgetown, ON) and Thermo Fisher Scientific (Whitby, ON). Dibenzylcyclooctyne-NHS ester (DBCO-NHS) was gifted by Dr Alex Adronov at McMaster University (Hamilton, ON). | Get A Quote |
Degradable low-fouling hydrogels are ideal vehicles for drug and cell delivery. For each application, hydrogel degradation rate must be re-optimized for maximum therapeutic benefit. We developed a method to rapidly and predictably tune degradation rates of low-fouling poly(oligo(ethylene glycol)methyl ether methacrylate) (P(EG)xMA) hydrogels by modifying two interdependent variables: (1) base-catalysed crosslink degradation kinetics, dependent on crosslinker electronics (electron withdrawing groups (EWGs)); and, (2) polymer hydration, dependent on the molecular weight (MW) of poly(ethylene glycol) (PEG) pendant groups. By controlling PEG MW and EWG strength, P(EG)xMA hydrogels were tuned to degrade over 6 to ... More
