| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | EF1-tagged genes EF1-N-MACIR and EF1-C-MACIR were prepared by PCR. The forward primer: 5’-GGATCCGAAGTCGATATTAATG-3’ included a BamHI restriction site. The reverse primer: 5’-AAGCTTTGTATTATTCCTAGC-3’ included a HindIII restriction site for cloning into the expression vectors pEF1N-CMV and pEF1C-CMV. FLAG-tagged MACIR wt, P116A/Y117Q and P142A/Y143Q mutants with a C terminal FLAG tag were synthesized by Genscript (genscript.com). | Get A Quote |
Expression of the macrophage immunometabolism regulator gene (MACIR) is associated with severity of autoimmune disease pathology and the regulation of macrophage biology through unknown mechanisms. The 206 amino acid protein lacks homology to any characterized protein sequence and is a disordered protein according to structure prediction algorithms. Here we identify specific interactions of MACIR using a fragment complementation-based affinity pull down of cellular proteins prepared with a membrane solubilization buffer. Quantitative mass spectrometry showed enrichment of nuclear and mitochondrial proteins and of 63 significant interacting proteins, binding to the nuclear transport receptor TNPO1 and traffickin... More
